Amino Acid Side-Chain Properties Flashcards: Polarity & Charge
56 flashcards on amino acid side-chain groups, charge at pH 7, and common property distinctions, with supplied-pKa reasoning and key exceptions.
A pakliról
Practice amino acid side-chain properties with 56 original flashcards for learners who already recognize the 20 standard amino-acid names. The cards ask you to recall a named amino acid’s property group, identify an amino acid or a specified set from a functional-group clue, distinguish commonly confused properties, and infer predominant side-chain charge from supplied pH and pKa values.
The reference pH is 7. Charge means the R group alone, excluding backbone groups and peptide termini. Ordinary charge statements use a simple aqueous reference model, not a guarantee about a particular site inside a protein. The calculations supply their pKa assumptions; one cysteine example changes the pH to 9.3, and one histidine example supplies a shifted local pKa. You do not need to memorize exact pKa values.
This deck uses a four-group study convention: nonpolar (glycine, alanine, valine, leucine, isoleucine, methionine, proline, phenylalanine, tryptophan); polar and predominantly uncharged at pH 7 (serine, threonine, asparagine, glutamine, cysteine, tyrosine); acidic (aspartate, glutamate); and basic (lysine, arginine, histidine). These are broad labels. Tryptophan has a polar hydrogen-bond donor; cysteine can behave hydrophobically; tyrosine is both aromatic and polar. Histidine belongs to the basic family but is mostly neutral at pH 7 when its side-chain pKa is 6.0. Aromaticity, polarity, and charge are not mutually exclusive descriptors.
The sequence starts with charge scope and classification, introduces the 20 amino acids in an interleaved order, then moves through property clues, comparisons, and pH reasoning. Related recall directions are separated so a nearby answer is less likely to give away the next one. Glycine’s flexibility, proline’s ring and backbone donor exception, cysteine’s disulfides, aromatic side chains, and acid-versus-amide distinctions receive focused practice.
The deck does not test one-letter or three-letter codes, structure drawings, exact pKa memorization, pI calculations, codons, dietary requirements, clinical decisions, or full-course/exam coverage. Broad clues are only reversed when the requested answer is a clear amino acid or an explicitly requested set. Structure and code recognition are covered separately in the amino acid structures and codes deck.
The questions, answers, examples, sequence, and metadata were independently authored from common biochemical facts. Fact checks used EMBL-EBI’s side-chain chemistry and ionisation guidance, plus ChEBI’s chemical classification. No source passages, exercises, or diagrams were copied. The cover is an original AI-generated conceptual illustration, not a chemical model.
Original text, organization, metadata, and generated cover are offered under CC0 1.0 to the extent applicable rights exist. This dedication does not cover source materials or anyone else’s rights. This is an independent study resource, not affiliated with or endorsed by EMBL-EBI or any course or examination provider.
Kártyák ebben a pakliban
1. kártya
Kérdés
When a card asks for an amino acid’s side-chain charge, which charges should you leave out?
Válasz
The backbone amino and carboxyl groups, including peptide termini. Count only the R group’s charge.
2. kártya
Kérdés
Can an uncharged amino-acid side chain still be polar?
Válasz
Yes. Uneven electron distribution can make a group polar without giving it a net charge; a hydroxyl group is an example.
3. kártya
Kérdés
Are ‘aromatic,’ ‘polar,’ and ‘basic’ mutually exclusive amino-acid labels?
Válasz
No. They describe different features. A ring can be aromatic while another feature makes the side chain polar or basic. Group labels are a study convention, not an exhaustive chemical description.
4. kártya
Kérdés
Alanine side chain → polarity group?
Válasz
Nonpolar. Its methyl group has no polar functional group.
5. kártya
Kérdés
Serine side chain at pH 7 → polarity/charge group?
Válasz
Polar, predominantly uncharged. It contains a hydroxyl group.
6. kártya
Kérdés
Aspartate side chain at pH 7 → acid/base group and predominant charge?
Válasz
Acidic; −1. The side-chain carboxyl group is predominantly deprotonated under the usual aqueous reference conditions.
7. kártya
Kérdés
Valine side chain → polarity group?
Válasz
Nonpolar. It is a branched hydrocarbon side chain.
8. kártya
Kérdés
Glutamine side chain at pH 7 → polarity/charge group?
Válasz
Polar, uncharged. Its amide group does not behave like glutamate’s carboxylate.
9. kártya
Kérdés
Lysine side chain at pH 7 → acid/base group and predominant charge?
Válasz
Basic; +1. Its terminal side-chain amino group is predominantly protonated under the usual aqueous reference conditions.
10. kártya
Kérdés
Phenylalanine side chain → polarity group?
Válasz
Nonpolar. Its benzyl group contains an aromatic ring.
11. kártya
Kérdés
Glycine → polarity group in this deck’s convention?
Válasz
Nonpolar. Its R group is just hydrogen; the grouping does not imply a large hydrophobic side chain.
12. kártya
Kérdés
Threonine side chain at pH 7 → polarity/charge group?
Válasz
Polar, predominantly uncharged. Its hydroxyl group contributes polarity despite the methyl group beside it.
13. kártya
Kérdés
Glutamate side chain at pH 7 → acid/base group and predominant charge?
Válasz
Acidic; −1. Its side-chain carboxyl group is predominantly deprotonated under the usual aqueous reference conditions.
14. kártya
Kérdés
Leucine side chain → polarity group?
Válasz
Nonpolar. It is a branched hydrocarbon side chain.
15. kártya
Kérdés
Cysteine at pH 7 → group in this deck’s convention?
Válasz
Polar, predominantly uncharged. The side-chain thiol is usually mostly protonated. Cysteine can also behave hydrophobically, so classification schemes differ.
16. kártya
Kérdés
Arginine side chain at pH 7 → acid/base group and predominant charge?
Válasz
Basic; +1. The guanidinium group is predominantly protonated under the usual aqueous reference conditions.
17. kártya
Kérdés
Tryptophan → polarity group in this deck’s convention?
Válasz
Nonpolar overall, with a polar feature. Its large aromatic indole group is hydrophobic, but its N–H can donate a hydrogen bond.
18. kártya
Kérdés
Proline → polarity group in this deck’s convention?
Válasz
Nonpolar. Its side chain also closes a ring with the backbone nitrogen, restricting backbone motion.
19. kártya
Kérdés
Asparagine side chain at pH 7 → polarity/charge group?
Válasz
Polar, uncharged. Its amide group is distinct from aspartate’s carboxylate.
20. kártya
Kérdés
Isoleucine side chain → polarity group?
Válasz
Nonpolar. It is a branched hydrocarbon side chain.
21. kártya
Kérdés
Histidine → acid/base family?
Válasz
Basic. Its imidazole can accept a proton. ‘Basic’ does not mean mostly +1 at pH 7: with a side-chain pKa of 6.0, most molecules have a neutral side chain.
22. kártya
Kérdés
Tyrosine at pH 7 → group in this deck’s convention?
Válasz
Polar, predominantly uncharged. Its phenolic hydroxyl adds polarity to an aromatic, partly hydrophobic side chain; other schemes group it with the aromatics.
23. kártya
Kérdés
Methionine side chain → polarity group?
Válasz
Nonpolar overall. Its sulfur is part of a thioether, not a thiol.
24. kártya
Kérdés
Which standard amino acid has hydrogen as its R group and no chiral alpha carbon?
Válasz
Glycine. Its small R group also permits more backbone conformations than most residues.
25. kártya
Kérdés
Which two standard amino acids have side-chain amide groups?
Válasz
Asparagine and glutamine. Their amides are polar and uncharged at pH 7.
26. kártya
Kérdés
For a single ionizable side-chain group, what does pH below its pKa favor?
Válasz
The protonated form. Whether that form is neutral or positive depends on the group.
27. kártya
Kérdés
Which two standard amino acids have aliphatic alcohol side chains?
Válasz
Serine and threonine. Tyrosine’s hydroxyl is phenolic, attached directly to an aromatic ring.
28. kártya
Kérdés
Which standard amino acid supplies the side-chain sulfur atoms of a protein disulfide bond?
Válasz
Cysteine. Oxidation of two cysteine thiols can produce a covalent S–S link.
29. kártya
Kérdés
Which three standard amino acids are conventionally called the branched-chain amino acids?
Válasz
Valine, leucine, and isoleucine. All three have nonpolar hydrocarbon side chains.
30. kártya
Kérdés
Which two standard amino acids usually have negatively charged side-chain carboxylates at pH 7?
Válasz
Aspartate and glutamate.
31. kártya
Kérdés
Which standard amino acid closes its side chain back onto the backbone nitrogen?
Válasz
Proline. The ring restricts backbone flexibility; an internal proline residue also lacks the usual peptide N–H donor.
32. kártya
Kérdés
For a single ionizable side-chain group, what is the protonated:deprotonated ratio at pH = pKa?
Válasz
1:1. Half is protonated and half is deprotonated in the simple two-state acid–base model.
33. kártya
Kérdés
Which three standard amino acids form the usual ‘aromatic amino acids’ study group?
Válasz
Phenylalanine, tyrosine, and tryptophan. Histidine’s imidazole is chemically aromatic too, but introductory grouping tables usually place histidine with the basic amino acids.
34. kártya
Kérdés
Cysteine versus methionine: which side chain can make the usual protein disulfide link?
Válasz
Cysteine. It has a thiol; methionine has a thioether and does not form this cysteine-type disulfide link.
35. kártya
Kérdés
For a single ionizable side-chain group, what does pH above its pKa favor?
Válasz
The deprotonated form. For a carboxyl group this is negative; for a protonated amine losing its proton, it is neutral.
36. kártya
Kérdés
At pH 7, why is aspartate usually charged while asparagine is uncharged?
Válasz
Aspartate has a side-chain carboxylate (−1); asparagine has a neutral amide. Similar names do not mean the same functional group.
37. kártya
Kérdés
Which standard amino acid has an imidazole side chain that can switch between neutral and +1 forms?
Válasz
Histidine. Its protonation depends on pH and its local pKa.
38. kártya
Kérdés
Which standard amino acid combines a phenyl ring with a phenolic hydroxyl group?
Válasz
Tyrosine. The hydroxyl makes this aromatic side chain more polar than phenylalanine’s.
39. kártya
Kérdés
Glycine versus proline: which generally allows more backbone conformations?
Válasz
Glycine. Its tiny R group imposes little steric restriction; proline’s ring constrains the backbone.
40. kártya
Kérdés
Why can tryptophan donate a side-chain hydrogen bond despite being grouped as nonpolar overall?
Válasz
Its indole N–H can donate a hydrogen bond. A large hydrophobic group can still contain a polar site.
41. kártya
Kérdés
Must every nonpolar side chain lie inside a water-soluble globular protein?
Válasz
No. Burial of nonpolar groups is a tendency, not a rule for every residue. Protein shape and local interactions also matter.
42. kártya
Kérdés
Assume histidine’s side-chain pKa is 6.0. At pH 7.0, is its predominant side-chain charge 0 or +1?
Válasz
0
pH is one unit above pKa, so neutral:protonated is 10:1; about 9% is +1. This is a supplied-pKa model, not a universal protein value.
43. kártya
Kérdés
Assume cysteine’s thiol pKa is 8.3. At pH 7.0, which form predominates: neutral thiol or negative thiolate?
Válasz
Neutral thiol. pH is below pKa, so the protonated S–H form predominates.
44. kártya
Kérdés
At pH 7, which has a predominantly −1 side chain: glutamate or glutamine?
Válasz
Glutamate. Glutamine’s side-chain amide is neutral.
45. kártya
Kérdés
Assume tyrosine’s phenolic pKa is 10.1. At pH 7.0, is its side chain predominantly neutral or negative?
Válasz
Neutral. The phenolic hydroxyl is mostly protonated because pH is below pKa.
46. kártya
Kérdés
Assume lysine’s side-chain pKa is 10.5. At pH 7.0, is its side chain predominantly neutral or +1?
Válasz
+1. pH is below pKa, favoring the protonated amino group.
47. kártya
Kérdés
Assume aspartate’s side-chain pKa is 3.9. At pH 7.0, is its side chain predominantly neutral or −1?
Válasz
−1. pH is above pKa, favoring the deprotonated carboxylate.
48. kártya
Kérdés
Can a standard aqueous pKa value determine a buried protein side chain’s charge with certainty?
Válasz
No. Nearby charges, solvent exposure, and other local interactions can shift pKa. Use a value appropriate to that site when it is known.
49. kártya
Kérdés
With a histidine side-chain pKa of 6.0, does the label ‘basic amino acid’ imply a predominantly +1 side chain at pH 7?
Válasz
No. ‘Basic’ describes proton-accepting chemistry; the predominant charge at a stated pH follows the pKa. Here the side chain is mostly neutral.
50. kártya
Kérdés
Does a predominantly neutral tyrosine side chain at pH 7 make tyrosine nonpolar in this deck’s grouping?
Válasz
No. Its hydroxyl is polar even when uncharged. This deck groups tyrosine as polar, uncharged and also recognizes its aromatic character.
51. kártya
Kérdés
Does an internal proline residue have the same backbone N–H hydrogen-bond donor as most peptide residues?
Válasz
No. Its backbone nitrogen has no attached hydrogen in the peptide chain. This is a backbone consequence of its ring, not a side-chain charge.
52. kártya
Kérdés
At pH = pKa, a side chain switches between 0 and +1. Is each individual side chain charged +0.5?
Válasz
No. The two forms occur in equal proportions, giving an ensemble-average charge of +0.5. Each form still has charge 0 or +1.
53. kártya
Kérdés
Assume cysteine’s thiol pKa is 8.3. At pH 9.3, what is its predominant side-chain charge?
Válasz
−1. Deprotonated thiolate predominates; the thiolate:thiol ratio is 10:1 in the simple model.
54. kártya
Kérdés
At pH 7, which pair is a plausible side-chain salt bridge: lysine–glutamate or leucine–valine?
Válasz
Lysine–glutamate: their usual side-chain charges are +1 and −1. A salt bridge still requires suitable proximity and environment.
55. kártya
Kérdés
A histidine site has a measured side-chain pKa of 8.0. At pH 7.0, which side-chain form predominates?
Válasz
The protonated +1 form. pH is below this site’s pKa; use the supplied local value rather than a generic histidine value.
56. kártya
Kérdés
Phenylalanine versus tyrosine: which has the side-chain hydroxyl that adds polarity?
Válasz
Tyrosine. Phenylalanine lacks that hydroxyl; both contain an aromatic ring.
56 kártya
Amino Acid Side-Chain Properties Flashcards: Polarity & Charge
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