DNA Reverse Complement Flashcards

הכרטיסים בחפיסה הזו

1. כרטיס 1

   שאלה

   In double-stranded DNA, which bases pair with adenine (A) and cytosine (C), respectively?

   תשובה

   Thymine (T) pairs with A. Guanine (G) pairs with C.

2. כרטיס 2

   שאלה

   Two DNA strands are aligned base by base. The top strand has its 5′ end on the left. Which end of the bottom strand is on the left?

   תשובה

   The 3′ end. The strands are antiparallel, so their directions are opposite.

3. כרטיס 3

   שאלה

   Write the complementary DNA strand directly below 5′–CATGGA–3′, pairing the bases in the same columns. Label its ends.

   תשובה

   3′–GTACCT–5′

   The aligned partner runs 3′ to 5′ from left to right.

4. כרטיס 4

   שאלה

   A DNA strand is written 5′ to 3′. How do you get its reverse complement?

   תשובה

   Pair each base, then read the paired bases in reverse order. The result gives the partner strand from its 5′ end to its 3′ end.

5. כרטיס 5

   שאלה

   What is the reverse complement of 5′–GTTCA–3′?

   תשובה

   5′–TGAAC–3′

   The aligned partner is 3′–CAAGT–5′. Read it from the right.

6. כרטיס 6

   שאלה

   Which two bases fill the blanks in the bottom DNA strand, from left to right?

   5′–G C T A G–3′
   3′–C _ A _ C–5′
   

   תשובה

   G, then T. They pair with C and A in the top strand.

   The completed bottom strand is 3′–CGATC–5′.

7. כרטיס 7

   שאלה

   Write the same DNA strand 5′ to 3′ if it is shown as 3′–TGGAC–5′.

   תשובה

   5′–CAGGT–3′

   Start from the right. The bases stay the same because you're reading the same strand.

8. כרטיס 8

   שאלה

   Write the strand that pairs with 3′–ACCTGA–5′. Give your answer 5′ to 3′.

   תשובה

   5′–TGGACT–3′

   The aligned partner already has its 5′ end on the left.

9. כרטיס 9

   שאלה

   Someone gives CGTGAC as the reverse complement of 5′–CAGTGC–3′. What step did they miss?

   תשובה

   They still need to complement each base. CGTGAC is the original sequence read backward. The reverse complement is 5′–GCACTG–3′.

10. כרטיס 10

    שאלה

    A DNA strand ends with G at its 3′ end. What is the first base of its reverse complement, written 5′ to 3′?

    תשובה

    C. The original 3′ end lines up with the partner's 5′ end, and C pairs with G.

11. כרטיס 11

    שאלה

    Write the reverse complement of 5′–ACGCTAA–3′.

    תשובה

    5′–TTAGCGT–3′

    Pair the bases to get 3′–TGCGATT–5′, then read from the right.

12. כרטיס 12

    שאלה

    Is 5′–AGCATGCT–3′ a reverse-complement palindrome?

    תשובה

    Yes. Its reverse complement is 5′–AGCATGCT–3′ again.

13. כרטיס 13

    שאלה

    Someone gives AGGCAT as the reverse complement of 5′–TCCGTA–3′. What step did they miss?

    תשובה

    They still need to reverse the order. AGGCAT is the aligned partner read 3′ to 5′. Read it from the other end to get 5′–TACGGA–3′.

14. כרטיס 14

    שאלה

    A DNA strand's reverse complement is 5′–CGAACTG–3′. What was the original sequence, written 5′ to 3′?

    תשובה

    5′–CAGTTCG–3′

    Taking the reverse complement a second time restores the original sequence.

15. כרטיס 15

    שאלה

    Find the exact + strand match to 5′–GATA–3′ on the printed + reference 5′–TCGGATACCAA–3′. Count the leftmost reference base as position 1 and include both ends of the match.

    תשובה

    Positions 4–7 (+). Those bases read GATA from left to right.

16. כרטיס 16

    שאלה

    You split a 5′→3′ DNA sequence into a left block and a right block. Which block provides the 5′ end of the reverse complement?

    תשובה

    The right block. The original 3′ end is on the right, across from the partner's 5′ end.

17. כרטיס 17

    שאלה

    An exact DNA query matches the + strand at reference positions 4–8. Which reference position holds the query's 5′ end?

    תשובה

    Position 4. The query runs left to right on the + strand.

18. כרטיס 18

    שאלה

    To find a − strand match to 5′–GCTAC–3′, what sequence should you look for on the printed 5′→3′ + reference?

    תשובה

    5′–GTAGC–3′

    That's the reverse complement of the query.

19. כרטיס 19

    שאלה

    Can a five-base DNA sequence made from A, C, G, and T equal its own reverse complement?

    תשובה

    No. Its middle base would have to pair with itself, and each of those four bases pairs with a different base.

20. כרטיס 20

    שאלה

    Search the printed + reference 5′–ACTAGGATTCCTAGT–3′ for 5′–CTAGG–3′ on either strand. Give each exact match's strand and 1-based inclusive reference positions.

    תשובה

    Positions 2–6 (+) and 10–14 (−).

    The reference has CTAGG at 2–6 and CCTAG, the query's reverse complement, at 10–14.

21. כרטיס 21

    שאלה

    One base in the bottom DNA strand is paired incorrectly. Which base should replace it, and at what position from the left?

    5′–A T C G C–3′
    3′–T A A C G–5′
    

    תשובה

    G at position 3. The top strand has C there.

    The corrected bottom strand is 3′–TAGCG–5′.

22. כרטיס 22

    שאלה

    A window on the printed + reference is 5′–CGAG–3′. Does its − strand exactly match the query 5′–CTCA–3′?

    תשובה

    No. The − strand at that span reads 5′–CTCG–3′. Its last base differs from the query.

23. כרטיס 23

    שאלה

    Find the exact − strand match to 5′–CCGA–3′ on the printed + reference 5′–GACTCGGAATC–3′. Give the 1-based inclusive reference positions.

    תשובה

    Positions 4–7 (−). The printed bases at 4–7 are TCGG. The opposite strand reads CCGA in the 5′→3′ direction.

24. כרטיס 24

    שאלה

    An exact DNA query matches the − strand at reference positions 6–10. Which reference position holds the query's 5′ end?

    תשובה

    Position 10. The query runs right to left on the − strand.

25. כרטיס 25

    שאלה

    A query of 5′–CGCG–3′ exactly matches the + strand at reference positions 3–6. How many strands match at that same span?

    תשובה

    Two. CGCG equals its own reverse complement, so positions 3–6 match on both strands.

25 כרטיסים

DNA Reverse Complement Flashcards

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