DNA Reverse Complement Flashcards

کارت‌های این دسته

1. کارت ۱

   پرسش

   In double-stranded DNA, which bases pair with adenine (A) and cytosine (C), respectively?

   پاسخ

   Thymine (T) pairs with A. Guanine (G) pairs with C.

2. کارت ۲

   پرسش

   Two DNA strands are aligned base by base. The top strand has its 5′ end on the left. Which end of the bottom strand is on the left?

   پاسخ

   The 3′ end. The strands are antiparallel, so their directions are opposite.

3. کارت ۳

   پرسش

   Write the complementary DNA strand directly below 5′–CATGGA–3′, pairing the bases in the same columns. Label its ends.

   پاسخ

   3′–GTACCT–5′

   The aligned partner runs 3′ to 5′ from left to right.

4. کارت ۴

   پرسش

   A DNA strand is written 5′ to 3′. How do you get its reverse complement?

   پاسخ

   Pair each base, then read the paired bases in reverse order. The result gives the partner strand from its 5′ end to its 3′ end.

5. کارت ۵

   پرسش

   What is the reverse complement of 5′–GTTCA–3′?

   پاسخ

   5′–TGAAC–3′

   The aligned partner is 3′–CAAGT–5′. Read it from the right.

6. کارت ۶

   پرسش

   Which two bases fill the blanks in the bottom DNA strand, from left to right?

   5′–G C T A G–3′
   3′–C _ A _ C–5′
   

   پاسخ

   G, then T. They pair with C and A in the top strand.

   The completed bottom strand is 3′–CGATC–5′.

7. کارت ۷

   پرسش

   Write the same DNA strand 5′ to 3′ if it is shown as 3′–TGGAC–5′.

   پاسخ

   5′–CAGGT–3′

   Start from the right. The bases stay the same because you're reading the same strand.

8. کارت ۸

   پرسش

   Write the strand that pairs with 3′–ACCTGA–5′. Give your answer 5′ to 3′.

   پاسخ

   5′–TGGACT–3′

   The aligned partner already has its 5′ end on the left.

9. کارت ۹

   پرسش

   Someone gives CGTGAC as the reverse complement of 5′–CAGTGC–3′. What step did they miss?

   پاسخ

   They still need to complement each base. CGTGAC is the original sequence read backward. The reverse complement is 5′–GCACTG–3′.

10. کارت ۱۰

    پرسش

    A DNA strand ends with G at its 3′ end. What is the first base of its reverse complement, written 5′ to 3′?

    پاسخ

    C. The original 3′ end lines up with the partner's 5′ end, and C pairs with G.

11. کارت ۱۱

    پرسش

    Write the reverse complement of 5′–ACGCTAA–3′.

    پاسخ

    5′–TTAGCGT–3′

    Pair the bases to get 3′–TGCGATT–5′, then read from the right.

12. کارت ۱۲

    پرسش

    Is 5′–AGCATGCT–3′ a reverse-complement palindrome?

    پاسخ

    Yes. Its reverse complement is 5′–AGCATGCT–3′ again.

13. کارت ۱۳

    پرسش

    Someone gives AGGCAT as the reverse complement of 5′–TCCGTA–3′. What step did they miss?

    پاسخ

    They still need to reverse the order. AGGCAT is the aligned partner read 3′ to 5′. Read it from the other end to get 5′–TACGGA–3′.

14. کارت ۱۴

    پرسش

    A DNA strand's reverse complement is 5′–CGAACTG–3′. What was the original sequence, written 5′ to 3′?

    پاسخ

    5′–CAGTTCG–3′

    Taking the reverse complement a second time restores the original sequence.

15. کارت ۱۵

    پرسش

    Find the exact + strand match to 5′–GATA–3′ on the printed + reference 5′–TCGGATACCAA–3′. Count the leftmost reference base as position 1 and include both ends of the match.

    پاسخ

    Positions 4–7 (+). Those bases read GATA from left to right.

16. کارت ۱۶

    پرسش

    You split a 5′→3′ DNA sequence into a left block and a right block. Which block provides the 5′ end of the reverse complement?

    پاسخ

    The right block. The original 3′ end is on the right, across from the partner's 5′ end.

17. کارت ۱۷

    پرسش

    An exact DNA query matches the + strand at reference positions 4–8. Which reference position holds the query's 5′ end?

    پاسخ

    Position 4. The query runs left to right on the + strand.

18. کارت ۱۸

    پرسش

    To find a − strand match to 5′–GCTAC–3′, what sequence should you look for on the printed 5′→3′ + reference?

    پاسخ

    5′–GTAGC–3′

    That's the reverse complement of the query.

19. کارت ۱۹

    پرسش

    Can a five-base DNA sequence made from A, C, G, and T equal its own reverse complement?

    پاسخ

    No. Its middle base would have to pair with itself, and each of those four bases pairs with a different base.

20. کارت ۲۰

    پرسش

    Search the printed + reference 5′–ACTAGGATTCCTAGT–3′ for 5′–CTAGG–3′ on either strand. Give each exact match's strand and 1-based inclusive reference positions.

    پاسخ

    Positions 2–6 (+) and 10–14 (−).

    The reference has CTAGG at 2–6 and CCTAG, the query's reverse complement, at 10–14.

21. کارت ۲۱

    پرسش

    One base in the bottom DNA strand is paired incorrectly. Which base should replace it, and at what position from the left?

    5′–A T C G C–3′
    3′–T A A C G–5′
    

    پاسخ

    G at position 3. The top strand has C there.

    The corrected bottom strand is 3′–TAGCG–5′.

22. کارت ۲۲

    پرسش

    A window on the printed + reference is 5′–CGAG–3′. Does its − strand exactly match the query 5′–CTCA–3′?

    پاسخ

    No. The − strand at that span reads 5′–CTCG–3′. Its last base differs from the query.

23. کارت ۲۳

    پرسش

    Find the exact − strand match to 5′–CCGA–3′ on the printed + reference 5′–GACTCGGAATC–3′. Give the 1-based inclusive reference positions.

    پاسخ

    Positions 4–7 (−). The printed bases at 4–7 are TCGG. The opposite strand reads CCGA in the 5′→3′ direction.

24. کارت ۲۴

    پرسش

    An exact DNA query matches the − strand at reference positions 6–10. Which reference position holds the query's 5′ end?

    پاسخ

    Position 10. The query runs right to left on the − strand.

25. کارت ۲۵

    پرسش

    A query of 5′–CGCG–3′ exactly matches the + strand at reference positions 3–6. How many strands match at that same span?

    پاسخ

    Two. CGCG equals its own reverse complement, so positions 3–6 match on both strands.

۲۵ کارت

DNA Reverse Complement Flashcards

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