DNA Reverse Complement Flashcards
Practice finding DNA reverse complements, reading 5′ and 3′ labels, and locating exact matches on either DNA strand.
เกี่ยวกับชุดการ์ดนี้
Start by pairing DNA bases and labeling the ends of the complementary strand. Then find reverse complements, work backward to the original sequence, and spot when a wrong answer skipped the pairing or the reversal.
Use the same direction check to find exact matches on the + and − strands and give their positions on the printed reference. You’ll also see how a reverse-complement palindrome can match both strands at the same span.
Every sequence example is original and artificial. The cards use A, C, G, and T and exact matches so you can focus on strand direction. RNA, approximate searches, and primer design need additional rules and are outside the scope.
การ์ดในชุดการ์ดนี้
การ์ด 1
คำถาม
In double-stranded DNA, which bases pair with adenine (A) and cytosine (C), respectively?
คำตอบ
Thymine (T) pairs with A. Guanine (G) pairs with C.
การ์ด 2
คำถาม
Two DNA strands are aligned base by base. The top strand has its 5′ end on the left. Which end of the bottom strand is on the left?
คำตอบ
The 3′ end. The strands are antiparallel, so their directions are opposite.
การ์ด 3
คำถาม
Write the complementary DNA strand directly below
5′–CATGGA–3′, pairing the bases in the same columns. Label its ends.คำตอบ
3′–GTACCT–5′The aligned partner runs 3′ to 5′ from left to right.
การ์ด 4
คำถาม
A DNA strand is written 5′ to 3′. How do you get its reverse complement?
คำตอบ
Pair each base, then read the paired bases in reverse order. The result gives the partner strand from its 5′ end to its 3′ end.
การ์ด 5
คำถาม
What is the reverse complement of
5′–GTTCA–3′?คำตอบ
5′–TGAAC–3′The aligned partner is
3′–CAAGT–5′. Read it from the right.การ์ด 6
คำถาม
Which two bases fill the blanks in the bottom DNA strand, from left to right?
5′–G C T A G–3′ 3′–C _ A _ C–5′คำตอบ
G, then T. They pair with C and A in the top strand.
The completed bottom strand is
3′–CGATC–5′.การ์ด 7
คำถาม
Write the same DNA strand 5′ to 3′ if it is shown as
3′–TGGAC–5′.คำตอบ
5′–CAGGT–3′Start from the right. The bases stay the same because you're reading the same strand.
การ์ด 8
คำถาม
Write the strand that pairs with
3′–ACCTGA–5′. Give your answer 5′ to 3′.คำตอบ
5′–TGGACT–3′The aligned partner already has its 5′ end on the left.
การ์ด 9
คำถาม
Someone gives
CGTGACas the reverse complement of5′–CAGTGC–3′. What step did they miss?คำตอบ
They still need to complement each base.
CGTGACis the original sequence read backward. The reverse complement is5′–GCACTG–3′.การ์ด 10
คำถาม
A DNA strand ends with G at its 3′ end. What is the first base of its reverse complement, written 5′ to 3′?
คำตอบ
C. The original 3′ end lines up with the partner's 5′ end, and C pairs with G.
การ์ด 11
คำถาม
Write the reverse complement of
5′–ACGCTAA–3′.คำตอบ
5′–TTAGCGT–3′Pair the bases to get
3′–TGCGATT–5′, then read from the right.การ์ด 12
คำถาม
Is
5′–AGCATGCT–3′a reverse-complement palindrome?คำตอบ
Yes. Its reverse complement is
5′–AGCATGCT–3′again.การ์ด 13
คำถาม
Someone gives
AGGCATas the reverse complement of5′–TCCGTA–3′. What step did they miss?คำตอบ
They still need to reverse the order.
AGGCATis the aligned partner read 3′ to 5′. Read it from the other end to get5′–TACGGA–3′.การ์ด 14
คำถาม
A DNA strand's reverse complement is
5′–CGAACTG–3′. What was the original sequence, written 5′ to 3′?คำตอบ
5′–CAGTTCG–3′Taking the reverse complement a second time restores the original sequence.
การ์ด 15
คำถาม
Find the exact + strand match to
5′–GATA–3′on the printed + reference5′–TCGGATACCAA–3′. Count the leftmost reference base as position 1 and include both ends of the match.คำตอบ
Positions 4–7 (+). Those bases read
GATAfrom left to right.การ์ด 16
คำถาม
You split a 5′→3′ DNA sequence into a left block and a right block. Which block provides the 5′ end of the reverse complement?
คำตอบ
The right block. The original 3′ end is on the right, across from the partner's 5′ end.
การ์ด 17
คำถาม
An exact DNA query matches the + strand at reference positions 4–8. Which reference position holds the query's 5′ end?
คำตอบ
Position 4. The query runs left to right on the + strand.
การ์ด 18
คำถาม
To find a − strand match to
5′–GCTAC–3′, what sequence should you look for on the printed 5′→3′ + reference?คำตอบ
5′–GTAGC–3′That's the reverse complement of the query.
การ์ด 19
คำถาม
Can a five-base DNA sequence made from A, C, G, and T equal its own reverse complement?
คำตอบ
No. Its middle base would have to pair with itself, and each of those four bases pairs with a different base.
การ์ด 20
คำถาม
Search the printed + reference
5′–ACTAGGATTCCTAGT–3′for5′–CTAGG–3′on either strand. Give each exact match's strand and 1-based inclusive reference positions.คำตอบ
Positions 2–6 (+) and 10–14 (−).
The reference has
CTAGGat 2–6 andCCTAG, the query's reverse complement, at 10–14.การ์ด 21
คำถาม
One base in the bottom DNA strand is paired incorrectly. Which base should replace it, and at what position from the left?
5′–A T C G C–3′ 3′–T A A C G–5′คำตอบ
G at position 3. The top strand has C there.
The corrected bottom strand is
3′–TAGCG–5′.การ์ด 22
คำถาม
A window on the printed + reference is
5′–CGAG–3′. Does its − strand exactly match the query5′–CTCA–3′?คำตอบ
No. The − strand at that span reads
5′–CTCG–3′. Its last base differs from the query.การ์ด 23
คำถาม
Find the exact − strand match to
5′–CCGA–3′on the printed + reference5′–GACTCGGAATC–3′. Give the 1-based inclusive reference positions.คำตอบ
Positions 4–7 (−). The printed bases at 4–7 are
TCGG. The opposite strand readsCCGAin the 5′→3′ direction.การ์ด 24
คำถาม
An exact DNA query matches the − strand at reference positions 6–10. Which reference position holds the query's 5′ end?
คำตอบ
Position 10. The query runs right to left on the − strand.
การ์ด 25
คำถาม
A query of
5′–CGCG–3′exactly matches the + strand at reference positions 3–6. How many strands match at that same span?คำตอบ
Two.
CGCGequals its own reverse complement, so positions 3–6 match on both strands.
25 การ์ด
DNA Reverse Complement Flashcards
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