Coding vs Template Strand Flashcards: Transcription Practice
Practice 46 original English cards on coding and template DNA strands: four RNA-conversion cases, strand choice, two reverse mappings, repairs, and evidence limits.
Sobre este baralho
Practice coding vs template strand decisions with 46 original English flashcards for introductory biology. Each sequence exercise states the strand’s role for a gene and labels its 5′ and 3′ ends. Report the initial, unprocessed RNA segment before processing, written 5′ → 3′.
The deck includes 20 DNA-to-RNA exercises: five coding strands displayed 5′ → 3′, five coding strands displayed 3′ → 5′, five template strands displayed 3′ → 5′, and five template strands displayed 5′ → 3′. Eight complementary two-strand displays ask which strand is the template from RNA polymerase’s stated movement, with four cases in each movement direction. Two reverse exercises start from an initial RNA segment and request its corresponding coding or template DNA segment, both explicitly written 5′ → 3′. Eight repairs address wrong rules or proposed answers, and four evidence checks identify missing role, orientation, processing information, or transcription evidence.
Four foundation cards introduce the template role, RNA synthesis direction, antiparallel alignment, and RNA alphabet. The fixed order starts with the basic coding and template cases, then mixes orientations, strand choices, repairs, reverse mappings, and limits. Repeated conversion-operation families are separated by at least three intervening cards, including related repairs and reverse mappings. The review scheduler handles later spacing. Answers give the result first and a short reason; every card carries the transcription-strands tag.
All short base sequences are invented practice segments, not identified genes. Mature-mRNA prediction is excluded because processing information is not supplied; one card asks you to recognize that limit. Translation, codon tables, reading frames, mutations, promoter inference, species-specific mechanisms, and complete-course or exam alignment are outside the scope. Additional reverse-orientation permutations and arbitrary sequence-generation prompts are omitted to keep the retrieval tasks focused. For a longer explanation, read the coding vs template strand lesson.
The questions, answers, artificial sequences, organization, and metadata were written independently with AI assistance and checked against common reference facts. Factual references are NHGRI’s Finding Genes and RNA fact sheet. No competitor cards, textbook examples, examination questions, source prose, diagrams, or logos were copied. Reference materials retain their own rights; facts are not claimed as proprietary.
Original material, including the AI-generated decorative cover, is dedicated under CC0 1.0 to the extent applicable rights exist. The cover is an abstract illustration, not a molecular diagram. This is independent study material; NHGRI and other institutions do not endorse it.
Cartões deste baralho
Cartão 1
Pergunta
For a gene, which DNA strand does RNA polymerase use as its base-pairing guide?
Resposta
The template strand. Its bases determine the complementary RNA bases.
Cartão 2
Pergunta
In which direction does RNA polymerase build a new RNA strand?
Resposta
5′ → 3′. New nucleotides are added at the growing RNA strand’s 3′ end.
Cartão 3
Pergunta
How do the directions of an RNA segment and its paired DNA template align?
Resposta
They are antiparallel: an RNA segment runs 5′ → 3′ alongside a template segment running 3′ → 5′.
Cartão 4
Pergunta
Which four base letters belong in an ordinary RNA sequence?
Resposta
A, U, C, and G. RNA uses U (uracil) where the corresponding coding DNA has T (thymine).
Cartão 5
Pergunta
This gene’s transcribed DNA segment is shown as the coding strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Coding DNA: 5′-GTACCTGA-3′Resposta
RNA: 5′-GUACCUGA-3′Keep the coding strand’s order and replace T with U.
Cartão 6
Pergunta
This gene’s transcribed DNA segment is shown as the template strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Template DNA: 3′-CAGTTCGA-5′Resposta
RNA: 5′-GUCAAGCU-3′Complement the template in the displayed order: A → U, T → A, C → G, G → C.
Cartão 7
Pergunta
RNA polymerase moves left to right across this transcribed segment. Which DNA strand is the template for this gene?
Top: 3′-AGCTCAGG-5′ Bottom: 5′-TCGAGTCC-3′Resposta
Top strand. Along the stated movement, this strand is read 3′ → 5′.
Cartão 8
Pergunta
This gene’s transcribed DNA segment is shown as the template strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Template DNA: 5′-TGCAGATCCA-3′Resposta
RNA: 5′-UGGAUCUGCA-3′Reverse the displayed template, then complement it: A → U, T → A, C → G, G → C.
Cartão 9
Pergunta
This gene’s transcribed DNA segment is shown as the coding strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Coding DNA: 5′-CAGTACGTT-3′Resposta
RNA: 5′-CAGUACGUU-3′Keep the coding strand’s order and replace T with U.
Cartão 10
Pergunta
This gene’s transcribed DNA segment is shown as the coding strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Coding DNA: 3′-CTAGACCTGA-5′Resposta
RNA: 5′-AGUCCAGAUC-3′Read the coding strand from its 5′ end: reverse the displayed letters, then replace T with U.
Cartão 11
Pergunta
RNA polymerase moves right to left across this transcribed segment. Which DNA strand is the template for this gene?
Top: 3′-ATCGGTCA-5′ Bottom: 5′-TAGCCAGT-3′Resposta
Bottom strand. Along the stated movement, this strand is read 3′ → 5′.
Cartão 12
Pergunta
This gene’s transcribed DNA segment is shown as the template strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Template DNA: 3′-GCTACCATGT-5′Resposta
RNA: 5′-CGAUGGUACA-3′Complement the template in the displayed order: A → U, T → A, C → G, G → C.
Cartão 13
Pergunta
This gene’s transcribed DNA segment is shown as the template strand. The proposal complements its displayed bases, then changes the end labels without reversing the letters. Correct the initial, unprocessed RNA segment before processing, 5′ → 3′.
Template DNA: 5′-CGATTCAG-3′ Proposed RNA: 5′-GCUAAGUC-3′Resposta
RNA: 5′-CUGAAUCG-3′The complement in displayed order runs 3′ → 5′. Reverse its letters as well as its end labels to report it 5′ → 3′.
Cartão 14
Pergunta
This gene’s transcribed DNA segment is shown as the coding strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Coding DNA: 5′-GCTTAGACCA-3′Resposta
RNA: 5′-GCUUAGACCA-3′Keep the coding strand’s order and replace T with U.
Cartão 15
Pergunta
This gene’s transcribed DNA segment is shown as the coding strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Coding DNA: 3′-CGTTAAGC-5′Resposta
RNA: 5′-CGAAUUGC-3′Read the coding strand from its 5′ end: reverse the displayed letters, then replace T with U.
Cartão 16
Pergunta
RNA polymerase moves left to right across this transcribed segment. Which DNA strand is the template for this gene?
Top: 3′-GACTAGCA-5′ Bottom: 5′-CTGATCGT-3′Resposta
Top strand. Along the stated movement, this strand is read 3′ → 5′.
Cartão 17
Pergunta
This gene’s transcribed DNA segment is shown as the template strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Template DNA: 3′-ATCCGTGA-5′Resposta
RNA: 5′-UAGGCACU-3′Complement the template in the displayed order: A → U, T → A, C → G, G → C.
Cartão 18
Pergunta
This gene’s transcribed DNA segment is shown as the template strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Template DNA: 5′-TCGAATGC-3′Resposta
RNA: 5′-GCAUUCGA-3′Reverse the displayed template, then complement it: A → U, T → A, C → G, G → C.
Cartão 19
Pergunta
This initial, unprocessed RNA segment was transcribed from a gene before processing. Write the corresponding coding DNA segment 5′ → 3′.
RNA: 5′-CUAAGUGC-3′Resposta
Coding DNA: 5′-CTAAGTGC-3′Keep the RNA’s order and replace U with T.
Cartão 20
Pergunta
RNA polymerase moves right to left across this transcribed segment. Which DNA strand is the template for this gene?
Top: 3′-TACGAGGT-5′ Bottom: 5′-ATGCTCCA-3′Resposta
Bottom strand. Along the stated movement, this strand is read 3′ → 5′.
Cartão 21
Pergunta
The only evidence offered for choosing a gene’s template is that the top strand contains ATG. What is missing?
Top: 5′-ATGCTCAG-3′ Bottom: 3′-TACGAGTC-5′Resposta
Evidence of which strand is transcribed for this gene, such as RNA polymerase’s movement across the segment. ATG alone does not establish the strand’s role.
Cartão 22
Pergunta
This gene’s transcribed DNA segment is shown as the template strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Template DNA: 5′-ACTGGCATG-3′Resposta
RNA: 5′-CAUGCCAGU-3′Reverse the displayed template, then complement it: A → U, T → A, C → G, G → C.
Cartão 23
Pergunta
This gene’s transcribed DNA segment is shown as the coding strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Coding DNA: 5′-ACAGTTGC-3′Resposta
RNA: 5′-ACAGUUGC-3′Keep the coding strand’s order and replace T with U.
Cartão 24
Pergunta
This gene’s transcribed DNA segment is shown as the coding strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Coding DNA: 3′-GACTTACGA-5′Resposta
RNA: 5′-AGCAUUCAG-3′Read the coding strand from its 5′ end: reverse the displayed letters, then replace T with U.
Cartão 25
Pergunta
RNA polymerase moves right to left across this transcribed segment. Which DNA strand is the template for this gene?
Top: 5′-TCGACAGT-3′ Bottom: 3′-AGCTGTCA-5′Resposta
Top strand. Along the stated movement, this strand is read 3′ → 5′.
Cartão 26
Pergunta
This gene’s transcribed DNA segment is shown as the template strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Template DNA: 3′-TCAGGATCA-5′Resposta
RNA: 5′-AGUCCUAGU-3′Complement the template in the displayed order: A → U, T → A, C → G, G → C.
Cartão 27
Pergunta
This gene’s transcribed DNA segment is shown as the template strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Template DNA: 5′-AGTCACGA-3′Resposta
RNA: 5′-UCGUGACU-3′Reverse the displayed template, then complement it: A → U, T → A, C → G, G → C.
Cartão 28
Pergunta
This gene’s transcribed DNA segment is shown as the coding strand. Correct the proposed initial, unprocessed RNA segment before processing, 5′ → 3′.
Coding DNA: 5′-GATACCGT-3′ Proposed RNA: 5′-UGCCAUAG-3′Resposta
RNA: 5′-GAUACCGU-3′The coding strand is already displayed 5′ → 3′. Replace T with U without reversing its letters.
Cartão 29
Pergunta
This gene’s transcribed DNA segment is shown as the coding strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Coding DNA: 3′-TGACCTAG-5′Resposta
RNA: 5′-GAUCCAGU-3′Read the coding strand from its 5′ end: reverse the displayed letters, then replace T with U.
Cartão 30
Pergunta
This gene’s transcribed DNA segment is shown as the template strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Template DNA: 3′-GATCCAGT-5′Resposta
RNA: 5′-CUAGGUCA-3′Complement the template in the displayed order: A → U, T → A, C → G, G → C.
Cartão 31
Pergunta
RNA polymerase moves right to left across this transcribed segment. Which DNA strand is the template for this gene?
Top: 5′-CTTAGCGA-3′ Bottom: 3′-GAATCGCT-5′Resposta
Top strand. Along the stated movement, this strand is read 3′ → 5′.
Cartão 32
Pergunta
This gene’s transcribed DNA segment is shown as the template strand. Correct the proposed initial, unprocessed RNA segment before processing, 5′ → 3′.
Template DNA: 5′-ACGTTCGA-3′ Proposed RNA: 5′-UGCAAGCU-3′Resposta
RNA: 5′-UCGAACGU-3′Complementing the displayed template gives RNA in the opposite direction. Reverse that complement to report the RNA 5′ → 3′.
Cartão 33
Pergunta
This gene’s transcribed DNA segment is shown as the coding strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Coding DNA: 5′-TTGACGCA-3′Resposta
RNA: 5′-UUGACGCA-3′Keep the coding strand’s order and replace T with U.
Cartão 34
Pergunta
Repair this rule: “The top DNA strand in a drawing is always the coding strand.”
Resposta
Top or bottom position does not assign a strand’s role for a gene. Use the stated role, or identify the template as the strand RNA polymerase reads 3′ → 5′ along its movement.
Cartão 35
Pergunta
This gene’s transcribed DNA segment is shown as the coding strand. What additional information is needed to infer the corresponding mature mRNA segment?
Coding DNA: 5′-ACCTGAGT-3′Resposta
Which processing changes, if any, affect this segment. Coding DNA alone determines the initial RNA sequence, but it does not tell you which bases remain in mature mRNA.
Cartão 36
Pergunta
This gene’s transcribed DNA segment is shown as the template strand. Correct the proposed initial, unprocessed RNA segment before processing, keeping the answer 5′ → 3′.
Template DNA: 3′-CATGGCAT-5′ Proposed RNA: 5′-CAUGGCAU-3′Resposta
RNA: 5′-GUACCGUA-3′The proposal copied the template. Pair each RNA base with the template base instead.
Cartão 37
Pergunta
This gene’s transcribed DNA segment is shown as the template strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Template DNA: 5′-GGCATCTA-3′Resposta
RNA: 5′-UAGAUGCC-3′Reverse the displayed template, then complement it: A → U, T → A, C → G, G → C.
Cartão 38
Pergunta
Repair this rule: “For a transcribed coding DNA segment already written 5′ → 3′, complement every base to get the initial RNA 5′ → 3′.”
Resposta
Keep the coding sequence’s letter order and replace T with U. The initial RNA is complementary to the template, so it matches the corresponding coding strand apart from U replacing T.
Cartão 39
Pergunta
Repair this rule: “Any DNA strand containing ATG must be the coding strand for the gene.”
Resposta
ATG alone does not identify the coding strand. You need the strand’s role for that gene or transcription-direction evidence; a short base pattern does not supply it.
Cartão 40
Pergunta
RNA polymerase moves left to right across this transcribed segment. Which DNA strand is the template for this gene?
Top: 5′-CGTAAGTC-3′ Bottom: 3′-GCATTCAG-5′Resposta
Bottom strand. Along the stated movement, this strand is read 3′ → 5′.
Cartão 41
Pergunta
A DNA segment is known to be transcribed, but its role is not given. What information is missing before you can assign one initial RNA segment?
DNA: 5′-CTGACATC-3′Resposta
Its role for this gene: coding or template. The end labels alone do not choose between copying the coding sequence and reverse-complementing the template.
Cartão 42
Pergunta
This gene’s transcribed DNA segment is shown as the coding strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Coding DNA: 3′-ATGCCAGT-5′Resposta
RNA: 5′-UGACCGUA-3′Read the coding strand from its 5′ end: reverse the displayed letters, then replace T with U.
Cartão 43
Pergunta
This initial, unprocessed RNA segment was transcribed from a gene before processing. Write the corresponding template DNA segment 5′ → 3′.
RNA: 5′-AGUCAGGA-3′Resposta
Template DNA: 5′-TCCTGACT-3′The paired template is 3′-TCAGTCCT-5′. Reverse it to report the DNA 5′ → 3′.
Cartão 44
Pergunta
This gene’s transcribed DNA segment is shown as the coding strand. Correct the proposed initial, unprocessed RNA segment before processing, 5′ → 3′.
Coding DNA: 5′-TGCAAGCT-3′ Proposed RNA: 5′-TGCAAGCT-3′Resposta
RNA: 5′-UGCAAGCU-3′Keep the coding strand’s order and replace both T bases with U.
Cartão 45
Pergunta
The shown coding DNA segment is transcribed for this gene. What is missing before you can write one initial, unprocessed RNA segment 5′ → 3′?
Coding DNA: GCATACCGResposta
The displayed direction: which end is 5′ and which is 3′. Without it, the letter order of the RNA written 5′ → 3′ is ambiguous.
Cartão 46
Pergunta
RNA polymerase moves left to right across this transcribed segment. Which DNA strand is the template for this gene?
Top: 5′-CAGTCGAT-3′ Bottom: 3′-GTCAGCTA-5′Resposta
Bottom strand. Along the stated movement, this strand is read 3′ → 5′.
46 cartões
Coding vs Template Strand Flashcards: Transcription Practice
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