Coding vs Template Strand Flashcards: Transcription Practice
Practice 46 original English cards on coding and template DNA strands: four RNA-conversion cases, strand choice, two reverse mappings, repairs, and evidence limits.
Despre acest pachet
Practice coding vs template strand decisions with 46 original English flashcards for introductory biology. Each sequence exercise states the strand’s role for a gene and labels its 5′ and 3′ ends. Report the initial, unprocessed RNA segment before processing, written 5′ → 3′.
The deck includes 20 DNA-to-RNA exercises: five coding strands displayed 5′ → 3′, five coding strands displayed 3′ → 5′, five template strands displayed 3′ → 5′, and five template strands displayed 5′ → 3′. Eight complementary two-strand displays ask which strand is the template from RNA polymerase’s stated movement, with four cases in each movement direction. Two reverse exercises start from an initial RNA segment and request its corresponding coding or template DNA segment, both explicitly written 5′ → 3′. Eight repairs address wrong rules or proposed answers, and four evidence checks identify missing role, orientation, processing information, or transcription evidence.
Four foundation cards introduce the template role, RNA synthesis direction, antiparallel alignment, and RNA alphabet. The fixed order starts with the basic coding and template cases, then mixes orientations, strand choices, repairs, reverse mappings, and limits. Repeated conversion-operation families are separated by at least three intervening cards, including related repairs and reverse mappings. The review scheduler handles later spacing. Answers give the result first and a short reason; every card carries the transcription-strands tag.
All short base sequences are invented practice segments, not identified genes. Mature-mRNA prediction is excluded because processing information is not supplied; one card asks you to recognize that limit. Translation, codon tables, reading frames, mutations, promoter inference, species-specific mechanisms, and complete-course or exam alignment are outside the scope. Additional reverse-orientation permutations and arbitrary sequence-generation prompts are omitted to keep the retrieval tasks focused. For a longer explanation, read the coding vs template strand lesson.
The questions, answers, artificial sequences, organization, and metadata were written independently with AI assistance and checked against common reference facts. Factual references are NHGRI’s Finding Genes and RNA fact sheet. No competitor cards, textbook examples, examination questions, source prose, diagrams, or logos were copied. Reference materials retain their own rights; facts are not claimed as proprietary.
Original material, including the AI-generated decorative cover, is dedicated under CC0 1.0 to the extent applicable rights exist. The cover is an abstract illustration, not a molecular diagram. This is independent study material; NHGRI and other institutions do not endorse it.
Fișele din acest pachet
Fișa 1
Întrebare
For a gene, which DNA strand does RNA polymerase use as its base-pairing guide?
Răspuns
The template strand. Its bases determine the complementary RNA bases.
Fișa 2
Întrebare
In which direction does RNA polymerase build a new RNA strand?
Răspuns
5′ → 3′. New nucleotides are added at the growing RNA strand’s 3′ end.
Fișa 3
Întrebare
How do the directions of an RNA segment and its paired DNA template align?
Răspuns
They are antiparallel: an RNA segment runs 5′ → 3′ alongside a template segment running 3′ → 5′.
Fișa 4
Întrebare
Which four base letters belong in an ordinary RNA sequence?
Răspuns
A, U, C, and G. RNA uses U (uracil) where the corresponding coding DNA has T (thymine).
Fișa 5
Întrebare
This gene’s transcribed DNA segment is shown as the coding strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Coding DNA: 5′-GTACCTGA-3′Răspuns
RNA: 5′-GUACCUGA-3′Keep the coding strand’s order and replace T with U.
Fișa 6
Întrebare
This gene’s transcribed DNA segment is shown as the template strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Template DNA: 3′-CAGTTCGA-5′Răspuns
RNA: 5′-GUCAAGCU-3′Complement the template in the displayed order: A → U, T → A, C → G, G → C.
Fișa 7
Întrebare
RNA polymerase moves left to right across this transcribed segment. Which DNA strand is the template for this gene?
Top: 3′-AGCTCAGG-5′ Bottom: 5′-TCGAGTCC-3′Răspuns
Top strand. Along the stated movement, this strand is read 3′ → 5′.
Fișa 8
Întrebare
This gene’s transcribed DNA segment is shown as the template strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Template DNA: 5′-TGCAGATCCA-3′Răspuns
RNA: 5′-UGGAUCUGCA-3′Reverse the displayed template, then complement it: A → U, T → A, C → G, G → C.
Fișa 9
Întrebare
This gene’s transcribed DNA segment is shown as the coding strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Coding DNA: 5′-CAGTACGTT-3′Răspuns
RNA: 5′-CAGUACGUU-3′Keep the coding strand’s order and replace T with U.
Fișa 10
Întrebare
This gene’s transcribed DNA segment is shown as the coding strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Coding DNA: 3′-CTAGACCTGA-5′Răspuns
RNA: 5′-AGUCCAGAUC-3′Read the coding strand from its 5′ end: reverse the displayed letters, then replace T with U.
Fișa 11
Întrebare
RNA polymerase moves right to left across this transcribed segment. Which DNA strand is the template for this gene?
Top: 3′-ATCGGTCA-5′ Bottom: 5′-TAGCCAGT-3′Răspuns
Bottom strand. Along the stated movement, this strand is read 3′ → 5′.
Fișa 12
Întrebare
This gene’s transcribed DNA segment is shown as the template strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Template DNA: 3′-GCTACCATGT-5′Răspuns
RNA: 5′-CGAUGGUACA-3′Complement the template in the displayed order: A → U, T → A, C → G, G → C.
Fișa 13
Întrebare
This gene’s transcribed DNA segment is shown as the template strand. The proposal complements its displayed bases, then changes the end labels without reversing the letters. Correct the initial, unprocessed RNA segment before processing, 5′ → 3′.
Template DNA: 5′-CGATTCAG-3′ Proposed RNA: 5′-GCUAAGUC-3′Răspuns
RNA: 5′-CUGAAUCG-3′The complement in displayed order runs 3′ → 5′. Reverse its letters as well as its end labels to report it 5′ → 3′.
Fișa 14
Întrebare
This gene’s transcribed DNA segment is shown as the coding strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Coding DNA: 5′-GCTTAGACCA-3′Răspuns
RNA: 5′-GCUUAGACCA-3′Keep the coding strand’s order and replace T with U.
Fișa 15
Întrebare
This gene’s transcribed DNA segment is shown as the coding strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Coding DNA: 3′-CGTTAAGC-5′Răspuns
RNA: 5′-CGAAUUGC-3′Read the coding strand from its 5′ end: reverse the displayed letters, then replace T with U.
Fișa 16
Întrebare
RNA polymerase moves left to right across this transcribed segment. Which DNA strand is the template for this gene?
Top: 3′-GACTAGCA-5′ Bottom: 5′-CTGATCGT-3′Răspuns
Top strand. Along the stated movement, this strand is read 3′ → 5′.
Fișa 17
Întrebare
This gene’s transcribed DNA segment is shown as the template strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Template DNA: 3′-ATCCGTGA-5′Răspuns
RNA: 5′-UAGGCACU-3′Complement the template in the displayed order: A → U, T → A, C → G, G → C.
Fișa 18
Întrebare
This gene’s transcribed DNA segment is shown as the template strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Template DNA: 5′-TCGAATGC-3′Răspuns
RNA: 5′-GCAUUCGA-3′Reverse the displayed template, then complement it: A → U, T → A, C → G, G → C.
Fișa 19
Întrebare
This initial, unprocessed RNA segment was transcribed from a gene before processing. Write the corresponding coding DNA segment 5′ → 3′.
RNA: 5′-CUAAGUGC-3′Răspuns
Coding DNA: 5′-CTAAGTGC-3′Keep the RNA’s order and replace U with T.
Fișa 20
Întrebare
RNA polymerase moves right to left across this transcribed segment. Which DNA strand is the template for this gene?
Top: 3′-TACGAGGT-5′ Bottom: 5′-ATGCTCCA-3′Răspuns
Bottom strand. Along the stated movement, this strand is read 3′ → 5′.
Fișa 21
Întrebare
The only evidence offered for choosing a gene’s template is that the top strand contains ATG. What is missing?
Top: 5′-ATGCTCAG-3′ Bottom: 3′-TACGAGTC-5′Răspuns
Evidence of which strand is transcribed for this gene, such as RNA polymerase’s movement across the segment. ATG alone does not establish the strand’s role.
Fișa 22
Întrebare
This gene’s transcribed DNA segment is shown as the template strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Template DNA: 5′-ACTGGCATG-3′Răspuns
RNA: 5′-CAUGCCAGU-3′Reverse the displayed template, then complement it: A → U, T → A, C → G, G → C.
Fișa 23
Întrebare
This gene’s transcribed DNA segment is shown as the coding strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Coding DNA: 5′-ACAGTTGC-3′Răspuns
RNA: 5′-ACAGUUGC-3′Keep the coding strand’s order and replace T with U.
Fișa 24
Întrebare
This gene’s transcribed DNA segment is shown as the coding strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Coding DNA: 3′-GACTTACGA-5′Răspuns
RNA: 5′-AGCAUUCAG-3′Read the coding strand from its 5′ end: reverse the displayed letters, then replace T with U.
Fișa 25
Întrebare
RNA polymerase moves right to left across this transcribed segment. Which DNA strand is the template for this gene?
Top: 5′-TCGACAGT-3′ Bottom: 3′-AGCTGTCA-5′Răspuns
Top strand. Along the stated movement, this strand is read 3′ → 5′.
Fișa 26
Întrebare
This gene’s transcribed DNA segment is shown as the template strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Template DNA: 3′-TCAGGATCA-5′Răspuns
RNA: 5′-AGUCCUAGU-3′Complement the template in the displayed order: A → U, T → A, C → G, G → C.
Fișa 27
Întrebare
This gene’s transcribed DNA segment is shown as the template strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Template DNA: 5′-AGTCACGA-3′Răspuns
RNA: 5′-UCGUGACU-3′Reverse the displayed template, then complement it: A → U, T → A, C → G, G → C.
Fișa 28
Întrebare
This gene’s transcribed DNA segment is shown as the coding strand. Correct the proposed initial, unprocessed RNA segment before processing, 5′ → 3′.
Coding DNA: 5′-GATACCGT-3′ Proposed RNA: 5′-UGCCAUAG-3′Răspuns
RNA: 5′-GAUACCGU-3′The coding strand is already displayed 5′ → 3′. Replace T with U without reversing its letters.
Fișa 29
Întrebare
This gene’s transcribed DNA segment is shown as the coding strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Coding DNA: 3′-TGACCTAG-5′Răspuns
RNA: 5′-GAUCCAGU-3′Read the coding strand from its 5′ end: reverse the displayed letters, then replace T with U.
Fișa 30
Întrebare
This gene’s transcribed DNA segment is shown as the template strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Template DNA: 3′-GATCCAGT-5′Răspuns
RNA: 5′-CUAGGUCA-3′Complement the template in the displayed order: A → U, T → A, C → G, G → C.
Fișa 31
Întrebare
RNA polymerase moves right to left across this transcribed segment. Which DNA strand is the template for this gene?
Top: 5′-CTTAGCGA-3′ Bottom: 3′-GAATCGCT-5′Răspuns
Top strand. Along the stated movement, this strand is read 3′ → 5′.
Fișa 32
Întrebare
This gene’s transcribed DNA segment is shown as the template strand. Correct the proposed initial, unprocessed RNA segment before processing, 5′ → 3′.
Template DNA: 5′-ACGTTCGA-3′ Proposed RNA: 5′-UGCAAGCU-3′Răspuns
RNA: 5′-UCGAACGU-3′Complementing the displayed template gives RNA in the opposite direction. Reverse that complement to report the RNA 5′ → 3′.
Fișa 33
Întrebare
This gene’s transcribed DNA segment is shown as the coding strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Coding DNA: 5′-TTGACGCA-3′Răspuns
RNA: 5′-UUGACGCA-3′Keep the coding strand’s order and replace T with U.
Fișa 34
Întrebare
Repair this rule: “The top DNA strand in a drawing is always the coding strand.”
Răspuns
Top or bottom position does not assign a strand’s role for a gene. Use the stated role, or identify the template as the strand RNA polymerase reads 3′ → 5′ along its movement.
Fișa 35
Întrebare
This gene’s transcribed DNA segment is shown as the coding strand. What additional information is needed to infer the corresponding mature mRNA segment?
Coding DNA: 5′-ACCTGAGT-3′Răspuns
Which processing changes, if any, affect this segment. Coding DNA alone determines the initial RNA sequence, but it does not tell you which bases remain in mature mRNA.
Fișa 36
Întrebare
This gene’s transcribed DNA segment is shown as the template strand. Correct the proposed initial, unprocessed RNA segment before processing, keeping the answer 5′ → 3′.
Template DNA: 3′-CATGGCAT-5′ Proposed RNA: 5′-CAUGGCAU-3′Răspuns
RNA: 5′-GUACCGUA-3′The proposal copied the template. Pair each RNA base with the template base instead.
Fișa 37
Întrebare
This gene’s transcribed DNA segment is shown as the template strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Template DNA: 5′-GGCATCTA-3′Răspuns
RNA: 5′-UAGAUGCC-3′Reverse the displayed template, then complement it: A → U, T → A, C → G, G → C.
Fișa 38
Întrebare
Repair this rule: “For a transcribed coding DNA segment already written 5′ → 3′, complement every base to get the initial RNA 5′ → 3′.”
Răspuns
Keep the coding sequence’s letter order and replace T with U. The initial RNA is complementary to the template, so it matches the corresponding coding strand apart from U replacing T.
Fișa 39
Întrebare
Repair this rule: “Any DNA strand containing ATG must be the coding strand for the gene.”
Răspuns
ATG alone does not identify the coding strand. You need the strand’s role for that gene or transcription-direction evidence; a short base pattern does not supply it.
Fișa 40
Întrebare
RNA polymerase moves left to right across this transcribed segment. Which DNA strand is the template for this gene?
Top: 5′-CGTAAGTC-3′ Bottom: 3′-GCATTCAG-5′Răspuns
Bottom strand. Along the stated movement, this strand is read 3′ → 5′.
Fișa 41
Întrebare
A DNA segment is known to be transcribed, but its role is not given. What information is missing before you can assign one initial RNA segment?
DNA: 5′-CTGACATC-3′Răspuns
Its role for this gene: coding or template. The end labels alone do not choose between copying the coding sequence and reverse-complementing the template.
Fișa 42
Întrebare
This gene’s transcribed DNA segment is shown as the coding strand. Write the initial, unprocessed RNA segment before processing, 5′ → 3′.
Coding DNA: 3′-ATGCCAGT-5′Răspuns
RNA: 5′-UGACCGUA-3′Read the coding strand from its 5′ end: reverse the displayed letters, then replace T with U.
Fișa 43
Întrebare
This initial, unprocessed RNA segment was transcribed from a gene before processing. Write the corresponding template DNA segment 5′ → 3′.
RNA: 5′-AGUCAGGA-3′Răspuns
Template DNA: 5′-TCCTGACT-3′The paired template is 3′-TCAGTCCT-5′. Reverse it to report the DNA 5′ → 3′.
Fișa 44
Întrebare
This gene’s transcribed DNA segment is shown as the coding strand. Correct the proposed initial, unprocessed RNA segment before processing, 5′ → 3′.
Coding DNA: 5′-TGCAAGCT-3′ Proposed RNA: 5′-TGCAAGCT-3′Răspuns
RNA: 5′-UGCAAGCU-3′Keep the coding strand’s order and replace both T bases with U.
Fișa 45
Întrebare
The shown coding DNA segment is transcribed for this gene. What is missing before you can write one initial, unprocessed RNA segment 5′ → 3′?
Coding DNA: GCATACCGRăspuns
The displayed direction: which end is 5′ and which is 3′. Without it, the letter order of the RNA written 5′ → 3′ is ambiguous.
Fișa 46
Întrebare
RNA polymerase moves left to right across this transcribed segment. Which DNA strand is the template for this gene?
Top: 5′-CAGTCGAT-3′ Bottom: 3′-GTCAGCTA-5′Răspuns
Bottom strand. Along the stated movement, this strand is read 3′ → 5′.
46 de fișe
Coding vs Template Strand Flashcards: Transcription Practice
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